Live adult male specimens of Anisogomphus bivittatus of family Gomphidae have been collected from Andretta, Himachal Pradesh (India). Male germ cell chromosomes of the species have been described on the basis of conventional staining, C-banding, silver nitrate staining and sequence specific staining. The species possesses 2n (♂) =23m, as the chromosome number and X0 (♂)/XX (♀) type sex determination. Dark terminal C-bands are present on all the autosomal bivalents, while m bivalent is C-negative and X chromosome is C-positive throughout the length. Terminal light/dark NOR’s are present on all autosomal bivalents including m bivalent, while X chromosome possesses terminal dark NOR on one side and light NOR on other side. During sequence specific staining, all the autosomal bivalents except m bivalent show more CMA₃ bright signals than DAPI signals at chiasmatic ends and X chromosome is also more CMA₃ bright than DAPI.