Toxic effects of acetamiprid on Apis mellifera were tested in the laboratory. Prepared solutions of commercial formulation (4.8 ng/µl, 10.65ng/µl, 21.42ng/µl, 51.52ng/µl, 113.4ng/µl) dispersed in honey syrup and tape water,were administered orally and applied topically on the thorax. Results show that acetamiprid is toxic to A. mellifera. Symptoms of neurotoxicity and the first cases of mortality appear 15 minutes after the ingestion of high concentrations and 30 minutes after the topical application of acetamiprid, and the mortality increases according to the concentration and time. LC50 values obtained after 24h are 5.6ng/μl for the topical application and 4.70 ng/μl for the oral route. Our results suggest a particular vulnerability of honey bee behavior to sublethal doses of acetamiprid. Overall, our results are valuable not only in evaluating the toxicity of acetamiprid onto honey bees but also in highlighting the proper application of pesticides for sustainable protection of bees and the environment.