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VOL. 11, ISSUE 2 (2026)
Antioxidant activity and mosquitocidal activity of Calophyllum inophyllum aqueous leaf against Aedes aegypti
Authors
Karupuswami Dhanalakshmi, Chellamuthu Vasugi
Abstract
The present study evaluated the antioxidant
and mosquitocidal activities of Calophyllum inophyllum aqueous
leaf extract against the dengue vector, Aedes aegypti. Antioxidant
potential was assessed using DPPH and ABTS radical scavenging assays, while
mosquitocidal efficacy was determined through ovicidal, larvicidal, and
pupicidal bioassays. The DPPH scavenging activity increased dose-dependently,
with percentage inhibition ranging from 22–28% at 100 µg/mL to 86–85% at 500
µg/mL, compared to 90–92% for ascorbic acid (standard). Similarly, ABTS
scavenging activity ranged from 16–18% at 100 µg/mL to 74–77% at 500 µg/mL,
with ascorbic acid showing 80–82% inhibition. These results indicate moderate
antioxidant capacity. For mosquitocidal activity, the extract exhibited
significant ovicidal effects, with egg hatchability decreasing from 97.8±1.92%
in controls to 84.0±1.87%, 54.2±1.78%, 24.2±1.92%, and 8.6±1.67% at 100, 200,
300, and 400 µg/mL, respectively; complete inhibition (NH) was observed at 500
µg/mL. Larvicidal and pupicidal assays revealed concentration-dependent
mortality across all instars and pupae. The 1st instar larvae showed the
highest susceptibility (LC₅₀ = 252.94 µg/mL; LC₉₀ = 455.39 µg/mL), while pupae
were most tolerant (LC₅₀ = 385.62 µg/mL; LC₉₀ = 665.83 µg/mL). Mortality rates
ranged from 7.2±1.92% (pupae at 100 µg/mL) to 97.0±1.30% (1st instar at 500
µg/mL). Regression equations showed positive concentration–response
relationships, and chi-square values indicated non-significant deviations (p
> 0.05). These findings suggest that C. inophyllum aqueous
leaf extract possesses promising antioxidant properties and potent
mosquitocidal activity, making it a potential eco-friendly alternative for
integrated vector management programs targeting A. aegypti. Further
studies on phytochemical characterization and field applicability are
warranted.
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Pages:548-553
How to cite this article:
Karupuswami Dhanalakshmi, Chellamuthu Vasugi "Antioxidant activity and mosquitocidal activity of <i>Calophyllum inophyllum</i> aqueous leaf against <i>Aedes aegypti</i>". International Journal of Entomology Research, Vol 11, Issue 2, 2026, Pages 548-553
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