Glyphosate-based herbicides (GBHs) are widely used in agriculture and may affect non-target organisms. Drosophila melanogaster is a well-established model organism for studying chemical-induced behavioural alterations. The present study evaluated the effect of GBH exposure on locomotor behaviour of Drosophila melanogaster.

Adult flies were exposed to different concentrations of GBH (5%, 10%, and 20%) through GBH treated culture medium for 7 days. Locomotor activity was assessed using a manual grid-based assay by recording the number of grid squares crossed during the observation period. The results showed a concentration-dependent decline in locomotor activity following GBH exposure. The 5% treated group showed a slight reduction in movement, whereas significant impairment was observed at higher concentrations, with the maximum reduction recorded in the 20% treated group. One-way ANOVA revealed significant differences among the experimental groups.